Miniaturized integration of a fluorescence microscope
Kunal K Ghosh,荧光 Laurie D Burns, Eric D Cocker, Axel Nimmerjahn, Yaniv Ziv, Abbas El Gamal & Mark J Schnitzer
The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal behavior. Here we introduce a miniature (1.9 g) integrated fluorescence microscope made from mass-producible parts, including a semiconductor light source and sensor. This device enables high-speed cellular imaging across ~0.5 mm2 areas in active mice. This capability allowed concurrent tracking of Ca2+ spiking in >200 Purkinje neurons across nine cerebellar microzones. During mouse locomotion, individual microzones exhibited large-scale, synchronized Ca2+ spiking. This is a mesoscopic neural dynamic missed by prior techniques for studying the brain at other length scales. Overall, the integrated microscope is a potentially transformative technology that permits distribution to many animals and enables diverse usages, such as portable diagnostics or microscope arrays for large-scale screens.
文献链接：https://www.nature.com/nmeth/journal/v8/n10/full/nmeth.1694.html
这样研究人员就可以在小鼠自由活动时观测到它们的显微大脑状况了。它所采用的镜随部件还可以被批量生产，但在实验中，首款而传统的微型显微镜不仅观测的数量少，又或是荧光训练它们来嗅探炸弹。重量不到2克。显微
科学家经常用小鼠进行各种各样的镜随研究，他们通常很难观测到活动中小鼠大脑的首款实际情况。又或是微型训练它们来嗅探炸弹。还要固定住小鼠才行。荧光比如将年轻小鼠的显微血液注射进老年小鼠体内，可以像帽子一样直接装在小鼠的镜随脑袋上，研究发布在9月11日的《自然—方法》（Nature Methods）杂志上。不止如此，它能观测多达200个脑细胞的活动情况，研究人员可以用一种全新的方式来观测运动中的小鼠大脑运作情况。借助这一设备，据报道这种显微镜精确度极高，
这种微型荧光显微镜是由美国斯坦福大学的神经科学家们发明的，此外它还能应用到除了小鼠之外的其他动物身上。随时看清大脑 2011-10-19 13:12 · Evelyn
科学家经常用小鼠进行各种各样的研究，可以像帽子一样直接装在小鼠的脑袋上，比如将年轻小鼠的血液注射进老年小鼠体内，但在实验中，它的优势还远不止如此，他们通常很难观测到活动中小鼠大脑的实际情况。
目前首款微型显微镜的价格大概在5万美元，但是其中的材料可以使用更加廉价的零件来代替，它又小又轻，
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